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Human ARSF ORF Clone in Gateway Cloning Vector

ORF Clon Gateway Cloning Vector pDONR223 Spectinomycin unconjugated

Catalog No. ABIN5321422

Quick Overview for Human ARSF ORF Clone in Gateway Cloning Vector (ABIN5321422)

Gene

ARSF (Arylsulfatase F (ARSF))

Application

Cloning (Clon)

Insert

ORF

Vector

Gateway Cloning Vector

Vector Backbone

pDONR223

Bacterial Resistance

Spectinomycin

Species

Human

Purpose

Entry clone of human cDNA (ORF) without STOP codon in Gateway pDONR223 Vector.

Sequence

ATGAGGCCCA GGAGACCCTT GGTCTTCATG TCTTTGGTGT GTGCACTCTT GAACACATGC CAGGCACACA GGGTGCATGA CGACAAGCCT AATATTGTCC TAATCATGGT TGATGACCTG GGTATTGGAG ATCTGGGCTG CTACGGCAAT GACACCATGA GGACGCCTCA CATCGACCGC CTTGCCAGGG AAGGCGTGCG ACTGACTCAG CACATCTCTG CCGCCTCCCT CTGCAGCCCA AGCCGGTCCG CGTTC

Components

2 μg of lyophilized gene in pDONR223 vector

Insert Length

1773 bp

Sequencing Primer

Forward primer M13R (5'→3'): GTAAAACGACGGCCAGT, Reverse primer T7P (5'→3'): TAATACGACTCACTATAGG
Human ARSF ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human ARSF cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Human ARSF CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human ARSF cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human ARSF cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human ARSF cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human ARSF cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human ARSF CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human ARSF CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human ARSF CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Restrictions

For Research Use only
Format

Lyophilized

Storage

-20 °C
Target

ARSF (Arylsulfatase F (ARSF))

Alternative Name

ARSF

Background

Arylsulfatase F

Gene ID

416

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