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Human CTSL2 ORF Clone in Gateway Cloning Vector

ORF Clon Gateway Cloning Vector pDONR223 Spectinomycin unconjugated

Catalog No. ABIN5324928

Quick Overview for Human CTSL2 ORF Clone in Gateway Cloning Vector (ABIN5324928)

Gene

Cathepsin L2 (CTSL2)

Application

Cloning (Clon)

Insert

ORF

Vector

Gateway Cloning Vector

Vector Backbone

pDONR223

Bacterial Resistance

Spectinomycin

Species

Human

Purpose

Entry clone of human cDNA (ORF) without STOP codon in Gateway pDONR223 Vector.

Sequence

ATGAATCTTT CGCTCGTCCT GGCTGCCTTT TGCTTGGGAA TAGCCTCCGC TGTTCCAAAA TTTGACCAAA ATTTGGATAC AAAGTGGTAC CAGTGGAAGG CAACACACAG AAGATTATAT GGCGCGAATG AAGAAGGATG GAGGAGAGCA GTGTGGGAAA AGAATATGAA AATGATTGAA CTGCACAATG GGGAATACAG CCAAGGGAAA CATGGCTTCA CAATGGCCAT GAATGCTTTT GGTGACATGA CCAAT

Components

2 μg of lyophilized gene in pDONR223 vector

Insert Length

1005 bp

Sequencing Primer

Forward primer M13R (5'→3'): GTAAAACGACGGCCAGT, Reverse primer T7P (5'→3'): TAATACGACTCACTATAGG
Human CTSL2 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human CTSL2 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human CTSL2 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human CTSL2 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Human CTSL2 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human CTSL2 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human CTSL2 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human CTSL2 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human CTSL2 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human CTSL2 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Restrictions

For Research Use only
Format

Lyophilized

Storage

-20 °C
Target

Cathepsin L2 (CTSL2)

Alternative Name

CTSL2

Background

Cathepsin L2

Gene ID

1515

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