Human TPI1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
This is a Triosephosphate Isomerase 1 plasmid from OriGene with ORF insert cloned into Mammalian Expression VectorpCMV6-Entry. Insert length: 861 bp. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin. Grade: End-sequenced.
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Catalog No. ABIN5376113
Supplier Product No.: rc228219
$445.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 16 to 17 Business Days
Quick Overview for Human TPI1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag) (ABIN5376113)
Gene
TPI1
(Triosephosphate Isomerase 1 (TPI1))
Fusion tag
Myc-DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Mammalian Expression Vector
Vector Backbone
pCMV6-Entry
Promoter
CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- rc228219
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Supplier
- OriGene
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Purpose
- Mammalian Vector with ORF clone of Human triosephosphate isomerase 1 (TPI1) transcript variant 2
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Specificity
- Restriction Site: SgfI-MluI
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Grade
- End-sequenced
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Components
- The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
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Insert Length
- 861 bp
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Sequencing Primer
- VP1.5 (forward) 5'GGACTTTCCAAAATGTCG 3', XL39 (reverse) 5'ATTAGGACAAGGCTGGTGGG 3'
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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- TPI1 (Triosephosphate Isomerase 1 (TPI1))
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Background
- This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
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NCBI Accession
- NM_001159287, NP_001152759
Target
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