Human FGF8 ORF Clone in Lentiviral Vector (Myc-DYKDDDDK Tag)
This is a Fibroblast Growth Factor 8 (Androgen-Induced) plasmid from OriGene with ORF insert cloned into Lentiviral VectorpLenti-C-Myc-DDK. Insert length: 615 bp. Transient expression. Suitable for PExp.
Bacterial selection: Chloramphenicol.
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Catalog No. ABIN5433181
Supplier Product No.: rc218442l1
$742.50
Plus shipping costs $50.00
10 μg
Shipping to:
United States
Delivery in 8 to 11 Business Days
Quick Overview for Human FGF8 ORF Clone in Lentiviral Vector (Myc-DYKDDDDK Tag) (ABIN5433181)
Gene
FGF8
(Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))
Fusion tag
Myc-DYKDDDDK Tag
Application
Protein Expression (PExp)
Insert
ORF
Vector
Lentiviral Vector
Vector Backbone
pLenti-C-Myc-DDK
Promoter
CMV Promoter
Bacterial Resistance
Chloramphenicol
Expression Type
Transient
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Species
- Human
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Supplier Product No.
- rc218442l1
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Supplier
- OriGene
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Purpose
- Lentiviral Vector with ORF clone of Human fibroblast growth factor 8 (androgen-induced) (FGF8) transcript variant A, C-term Myc-DDK-tagged
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Specificity
- Restriction Site: SgfI-MluI
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Characteristics
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Myc-DDK tagged, C-terminal
Broad cell spectrum: Lentivirus infect most cells, dividing & non-dividing, easy-to-transfect & hard-to-transfect cells.
High transduction efficiency
Convenience: Minimal need for optimization.
Safety: 3rd generation system with improved biosafety. -
Components
- 10 μg of lyophilized plasmid
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Insert Length
- 615 bp
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Application Notes
- In hard-to-transfect cells: Detection and purification of over-expressed protein
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- 4 °C/-20 °C
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- FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))
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Background
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.
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NCBI Accession
- NM_033165, NP_149355
Target
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