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Human RNF186 ORF Clone in Cloning Vector

ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Catalog No. ABIN5732474

Quick Overview for Human RNF186 ORF Clone in Cloning Vector (ABIN5732474)

Gene

RNF186 (Ring Finger Protein 186 (RNF186))

Application

Cloning (Clon)

Insert

ORF

Vector

Cloning Vector

Vector Backbone

pORF

Bacterial Resistance

Spectinomycin

Expression Type

Transient

Species

Human

Purpose

ORF Cloning-Vector holds the gene between an AflII and EcoRV cut site.

Insert Length

684 bp

Sequencing Primer

M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Human RNF186 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Mouse RNF186 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human RNF186 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human RNF186 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human RNF186 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human RNF186 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human RNF186 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Mouse RNF186 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Rat RNF186 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Mouse RNF186 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

10  mM Tris-HCI, 1  mM EDTA,  pH 8.0

Storage

-20 °C

Storage Comment

1 year when stored at -20°C or lower in a non-frost free freezer.
Target

RNF186 (Ring Finger Protein 186 (RNF186))

Alternative Name

RNF186

NCBI Accession

NM_019062

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