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Rat DDH ORF Clone in Cloning Vector

ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Catalog No. ABIN5739830

Quick Overview for Rat DDH ORF Clone in Cloning Vector (ABIN5739830)

Gene

AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Application

Cloning (Clon)

Insert

ORF

Vector

Cloning Vector

Vector Backbone

pORF

Bacterial Resistance

Spectinomycin

Expression Type

Transient

Species

Rat

Purpose

ORF Cloning-Vector holds the gene between an AflII and EcoRV cut site.

Insert Length

972 bp

Sequencing Primer

M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Human DDH ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human DDH cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Rat DDH CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human DDH CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human DDH cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human DDH cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Human DDH cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human DDH cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Rat DDH CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Human DDH CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

10  mM Tris-HCI, 1  mM EDTA,  pH 8.0

Storage

-20 °C

Storage Comment

1 year when stored at -20°C or lower in a non-frost free freezer.
Target

AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name

Akr1c1

NCBI Accession

NM_001033697

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