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Mouse TMEM258 ORF Clone in Cloning Vector

ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Catalog No. ABIN5768361

Quick Overview for Mouse TMEM258 ORF Clone in Cloning Vector (ABIN5768361)

Gene

TMEM258 (Transmembrane Protein 258 (TMEM258))

Application

Cloning (Clon)

Insert

ORF

Vector

Cloning Vector

Vector Backbone

pORF

Bacterial Resistance

Spectinomycin

Expression Type

Transient

Species

Mouse

Purpose

ORF Cloning-Vector holds the gene between an AflII and EcoRV cut site.

Insert Length

240 bp

Sequencing Primer

M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Human TMEM258 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human TMEM258 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Human TMEM258 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Mouse TMEM258 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Human TMEM258 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Human TMEM258 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Human TMEM258 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Human TMEM258 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Mouse TMEM258 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Human TMEM258 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

10  mM Tris-HCI, 1  mM EDTA,  pH 8.0

Storage

-20 °C

Storage Comment

1 year when stored at -20°C or lower in a non-frost free freezer.
Target

TMEM258 (Transmembrane Protein 258 (TMEM258))

Alternative Name

1810006K21Rik

NCBI Accession

NM_026919

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