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Rat RT1-DB1 ORF Clone in Cloning Vector

ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient

Catalog No. ABIN5770264

Quick Overview for Rat RT1-DB1 ORF Clone in Cloning Vector (ABIN5770264)

Gene

RT1-DB1 (RT1 Class II, Locus Db1 (RT1-DB1))

Application

Cloning (Clon)

Insert

ORF

Vector

Cloning Vector

Vector Backbone

pORF

Bacterial Resistance

Spectinomycin

Expression Type

Transient

Species

Rat

Purpose

ORF Cloning-Vector holds the gene between an AflII and EcoRV cut site.

Insert Length

795 bp

Sequencing Primer

M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Rat RT1-DB1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Rat RT1-DB1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Rat RT1-DB1 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable

Rat RT1-DB1 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient

Rat RT1-DB1 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable

Rat RT1-DB1 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient

Rat RT1-DB1 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient

Rat RT1-DB1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable

Rat RT1-DB1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable

Rat RT1-DB1 ORF Clone in Mammalian Expression Vector (Myc-DYKDDDDK Tag)
ORF PExp Mammalian Expression Vector pCMV6-Entry Kanamycin CMV Promoter Myc-DYKDDDDK Tag Transient

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Buffer

10  mM Tris-HCI, 1  mM EDTA,  pH 8.0

Storage

-20 °C

Storage Comment

1 year when stored at -20°C or lower in a non-frost free freezer.
Target

RT1-DB1 (RT1 Class II, Locus Db1 (RT1-DB1))

Alternative Name

RT1-Db1

NCBI Accession

NM_001008884

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