Human DOT1L cDNA Clone in Bacterial Expression Vector (His-MBP)
This is a DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) plasmid with cDNA insert cloned into Bacterial Expression VectorpPB-His-MBP. Insert length: not_set. Transient expression. Suitable for PExp.
Bacterial selection: Kanamycin.
Catalog No. ABIN5772689
$4,908.36
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500 ng
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United States
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Quick Overview for Human DOT1L cDNA Clone in Bacterial Expression Vector (His-MBP) (ABIN5772689)
Gene
DOT1L
(DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
Fusion tag
His-MBP
Application
Protein Expression (PExp)
Insert
cDNA
Vector
Bacterial Expression Vector
Vector Backbone
pPB-His-MBP
Promoter
T7 Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Human
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Purpose
- Prokaryotic expression plasmid for cDNA.
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Sequencing Primer
- MBP Forward primer: 5'-CGCAGATGTCCGCTTTCTGG-3', T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 10 mM Tris-HCI, 1 mM EDTA, pH 8.0
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Storage
- -20 °C
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Storage Comment
- 1 year when stored at -20°C or lower in a non-frost free freezer.
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- DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))
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Alternative Name
- DOT1L
Target
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