Casilio SgRNA-5xPBSc Mammalian Expression Vector
Casilio Regulation
EpEd
Mammalian Expression Vector
pX-SPFE-BbsI-sgRNA-5xPBSa
Ampicillin
U6 Promoter
unconjugated
Transient
Catalog No. ABIN7529438
Quick Overview for Casilio SgRNA-5xPBSc Mammalian Expression Vector (ABIN7529438)
Gene
SgRNA-5xPBSc
Application
Epigenetic Editing (EpEd)
Insert
Casilio Regulation
Vector
Mammalian Expression Vector
Vector Backbone
pX-SPFE-BbsI-sgRNA-5xPBSa
Promoter
U6 Promoter
Bacterial Resistance
Ampicillin
Expression Type
Transient
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Species
- Various Species
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Purpose
- Cloning vector for expression of Casilio sgRNA Containing five PUFca binding sites (PBSa). Customer sgRNA can be inserted by restriction cloning with BbsI.
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Sequencing Primer
- U6 fwd: GAGGGCCTATTTCCCATGATTCC Primer rev: GCCATTTGTCTGCAGAATTGGC
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 ng/μL
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Buffer
- 10 mM Tris-HCI,1 mM EDTA, pH 8.0
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Storage
- -20 °C
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Storage Comment
- 1 year when stored at -20°C or lower in a non-frost free freezer.
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- SgRNA-5xPBSc
Target
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