Casilio NLS-PUFc-VP64 Mammalian Expression Vector
This is a NLS-PUFc-VP64 plasmid with Casilio Regulation insert cloned into Mammalian Expression Vectorpmax-NLS-PUFc-VP64. Insert length: not_set. Transient expression. Suitable for EpEd.
Bacterial selection: Kanamycin.
Catalog No. ABIN7529444
$440.00
Plus shipping costs $50.00
50 ng
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Quick Overview for Casilio NLS-PUFc-VP64 Mammalian Expression Vector (ABIN7529444)
Gene
NLS-PUFc-VP64
Application
Epigenetic Editing (EpEd)
Insert
Casilio Regulation
Vector
Mammalian Expression Vector
Vector Backbone
pmax-NLS-PUFc-VP64
Promoter
CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Various Species
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Purpose
- Casilio mammalian expression vector for Pumilio RNA-Binding-Domain Type c and transcriptional activator VP64 under the control of a CAGGS + chim intron promoter. The ORF was inserted into pmax-DEST by Gateway recombination.
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Sequencing Primer
- Pum F1: GTATCTAGAGGCCGCAGCCGCCTTTTG, Pum R1: CCCTAAGTCAACACC
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 ng/μL
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Buffer
- 10 mM Tris-HCI,1 mM EDTA, pH 8.0
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Storage
- -20 °C
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Storage Comment
- 1 year when stored at -20°C or lower in a non-frost free freezer.
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- NLS-PUFc-VP64
Target
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