Casilio BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG Mammalian Expression Vector
Casilio Regulation
EpEd
Mammalian Expression Vector
pmax-BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
Kanamycin
CMV Promoter
BFP
Transient
Catalog No. ABIN7529446
Quick Overview for Casilio BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG Mammalian Expression Vector (ABIN7529446)
Gene
BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
Fusion tag
BFP
Application
Epigenetic Editing (EpEd)
Insert
Casilio Regulation
Vector
Mammalian Expression Vector
Vector Backbone
pmax-BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
Promoter
CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Various Species
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Purpose
- Casilio mammalian expression vector for Pumilio RNA-Binding-Domain Type a, several NLS, transcriptional regulator KRAB and BFP as marker under the control of a CAGGS + chim intron promoter. The ORF was inserted into pmax-DEST by Gateway recombination.
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Sequencing Primer
- Pum F1: GTATCTAGAGGCCGCAGCCGCCTTTTG, Pum R1: CCCTAAGTCAACACC
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 ng/μL
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Buffer
- 10 mM Tris-HCI,1 mM EDTA, pH 8.0
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Storage
- -20 °C
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Storage Comment
- 1 year when stored at -20°C or lower in a non-frost free freezer.
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- BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
Target
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