Casilio dCas9 Mammalian Expression Vector
Casilio Regulation
EpEd
Mammalian Expression Vector
pmax-dCas
Kanamycin
CMV Promoter
unconjugated
Transient
Catalog No. ABIN7529448
Quick Overview for Casilio dCas9 Mammalian Expression Vector (ABIN7529448)
Gene
dCas9
Application
Epigenetic Editing (EpEd)
Insert
Casilio Regulation
Vector
Mammalian Expression Vector
Vector Backbone
pmax-dCas
Promoter
CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
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Species
- Various Species
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Purpose
- Casilio mammalian expression vector for dCas9. In Casilio epigenetic editing experiments, this plasmid can be cotransfected to express dead Cas9 under control of the CAG promoter. The ORF was inserted into pmax-DEST by Gateway recombination.
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Sequencing Primer
- Primer for: GGGCTTGTCGAGACAGAGAAGAT Primer rev: ACCGAGGAGAGGGTTAGGGAT
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 ng/μL
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Buffer
- 10 mM Tris-HCI,1 mM EDTA, pH 8.0
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Storage
- -20 °C
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Storage Comment
- 1 year when stored at -20°C or lower in a non-frost free freezer.
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- dCas9
Target
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