This is a XRCC3_53BP1 plasmid with Casilio HDR insert cloned into Mammalian Expression Vectorpmax-XRCC3_53BP1. Insert length: not_set. Transient expression. Suitable for EpEd.
Bacterial selection: Kanamycin.
Quick Overview for Casilio XRCC3_53BP1 Mammalian Expression Vector (ABIN7536614)
Gene
XRCC3_53BP1
Application
Epigenetic Editing (EpEd)
Insert
Casilio HDR
Vector
Mammalian Expression Vector
Vector Backbone
pmax-XRCC3_53BP1
Promoter
CMV Promoter
Bacterial Resistance
Kanamycin
Expression Type
Transient
Species
Various Species
Purpose
Casilio mammalian expression vector for DNA double strand breaks-sensing 53BP1, several NLS and DNA repair protein XRCC3 under the control of a CAGGS + chim intron promoter. The ORF was inserted into pmax-DEST by Gateway recombination.
Sequencing Primer
Primer for: GGGCTTGTCGAGACAGAGAAGAT Primer rev: ACCGAGGAGAGGGTTAGGGAT
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 ng/μL
Buffer
10 mM Tris-HCI,1 mM EDTA, pH 8.0
Storage
-20 °C
Storage Comment
1 year when stored at -20°C or lower in a non-frost free freezer.