Casilio HDR
Genomics-online offers plasmids for HDR (homology directed repair) mediated gene editing. HDR-mediated gene editing can be applied for the gene knock-out, correction of point mutations and introduction of beneficial mutations. Our plasmids use the Casilio system which combines CRISPR/dCas9 with a Pumilio RNA-binding domain to increase specificity. The Pumilio binding site (PBS) recruits RNA-binding protein-tethered DNA repair proteins (DRP) to target site via aptamers appended to guide RNA. Casilio enables multimerization of multiple DRP. This minimizes errors in DNA repair, a crucial factor in in possible fields of application such as gene therapy.
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11 results :
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- CtIP(T847E)_53BP1
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-CtIP(T847E)_53BP1
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- CRISPR-Cas9
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-Cas9
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- FANCF_PUFa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-FANCF_PUFa
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- XRCC3_PUFa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-XRCC3_PUFa
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- BRCA1_PUFa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-BRCA1_PUFa
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- SgFE-EMX1-5xPBSa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgFE-EMX1-5xPBSa
- Resistance Gene:
- Spectinomycin
- Promoter:
- U6 Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- CtIP(T847E)_PUFa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmaxCtIP(T847E)_PUFa
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- XRCC3_53BP1
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-XRCC3_53BP1
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- BRCA1_53BP1
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-BRCA1_53BP1
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- FANCF_53BP1
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-FANCF_53BP1
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio HDR
- Application:
- Epigenetic Editing
- Gene:
- SgFE-CXCR4-5xPBSa
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgFE-CXCR4-5xPBSa
- Resistance Gene:
- Spectinomycin
- Promoter:
- U6 Promoter
- Expression:
- Transient