Casilio Imaging
genomics-online offers plasmids in order to facilitate the fluorescent detection of DNA-binding proteins. The plasmids use the Casilio system which is an improvement to conventional CRISPR-Cas. Casilio is a hybrid system combining CRISPR/dCas9 and Pumilio RNA-binding domain. The distinctive feature are repositioned effector proteins which enables multiplexing and multimerization. By inserting multiple binding sites and thus recruiting multiple molecules fluorescent proteins, the signal-to-noise ratio of in experiments like chromosome labeling is significantly lowered. Where conventional systems would require multiple experiments with subsequent analytical integration, Casilio can perform analysis in a single experiment.
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11 results :
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- dCas9
- Species:
- Various Species
- Vector type:
- Lentiviral Vector
- Vector backbone:
- lenti_dCas9_Blast
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Blasticidin
- Promoter:
- EFS Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- Clover_PUFc
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-Clover_PUFc
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- Clover_PUF48107
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-Clover_PUF48107
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Fusion Tag:
- GFP tag
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- iRFP670_PUFc
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-iRFP670_PUFc
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Fusion Tag:
- RFP tag
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- PUF9R_iRFP670
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-PUF9R_iRFP670
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Fusion Tag:
- RFP tag
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- PUF9R_mRuby2
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pmax-PUF9R_mRuby2
- Resistance Gene:
- Kanamycin
- Promoter:
- CMV Promoter
- Fusion Tag:
- RFP tag
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- SgRNA-15xPBSc
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgRNA-15xPBSc
- Resistance Gene:
- Spectinomycin
- Promoter:
- T7 Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- SgRNA-15xPBS9R
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgRNA-15xPBS9R
- Resistance Gene:
- Spectinomycin
- Promoter:
- T7 Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- SgRNA-20xPBS48107
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgRNA-20xPBS48107
- Resistance Gene:
- Spectinomycin
- Promoter:
- T7 Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- SgRNA-25xPBS9R
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgRNA-25xPBS9R
- Resistance Gene:
- Spectinomycin
- Promoter:
- T7 Promoter
- Expression:
- Transient
- Type:
- Casilio Imaging
- Application:
- Epigenetic Editing
- Gene:
- SgRNA-20xPBSc
- Species:
- Various Species
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCR8-sgRNA-20xPBSc
- Resistance Gene:
- Spectinomycin
- Promoter:
- T7 Promoter
- Expression:
- Transient