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Casilio Imaging

genomics-online offers plasmids in order to facilitate the fluorescent detection of DNA-binding proteins. The plasmids use the Casilio system which is an improvement to conventional CRISPR-Cas. Casilio is a hybrid system combining CRISPR/dCas9 and Pumilio RNA-binding domain. The distinctive feature are repositioned effector proteins which enables multiplexing and multimerization. By inserting multiple binding sites and thus recruiting multiple molecules fluorescent proteins, the signal-to-noise ratio of in experiments like chromosome labeling is significantly lowered. Where conventional systems would require multiple experiments with subsequent analytical integration, Casilio can perform analysis in a single experiment.

dCas9E1E1E1E1sgRNA
Fig.1: Multimerization in the Casilio imaging system. Recruiting multiple molecules fluorescent improves the signal strenght.

11 results :

Casilio Imaging EpEd Lentiviral Vector lenti_dCas9_Blast Ampicillin EFS Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
dCas9
Species:
Various Species
Vector type:
Lentiviral Vector
Vector backbone:
lenti_dCas9_Blast
Resistance Gene:
Ampicillin
Selection Marker:
Blasticidin
Promoter:
EFS Promoter
Expression:
Transient
Catalog No. ABIN7536597
Casilio Imaging EpEd Mammalian Expression Vector pmax-Clover_PUFc Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
Clover_PUFc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-Clover_PUFc
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7536598
Casilio Imaging EpEd Mammalian Expression Vector pmax-Clover_PUF48107 Kanamycin CMV Promoter GFP tag Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
Clover_PUF48107
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-Clover_PUF48107
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Fusion Tag:
GFP tag
Expression:
Transient
Catalog No. ABIN7536601
Casilio Imaging EpEd Mammalian Expression Vector pmax-iRFP670_PUFc Kanamycin CMV Promoter RFP tag Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
iRFP670_PUFc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-iRFP670_PUFc
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Fusion Tag:
RFP tag
Expression:
Transient
Catalog No. ABIN7536602
Casilio Imaging EpEd Mammalian Expression Vector pmax-PUF9R_iRFP670 Kanamycin CMV Promoter RFP tag Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
PUF9R_iRFP670
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-PUF9R_iRFP670
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Fusion Tag:
RFP tag
Expression:
Transient
Catalog No. ABIN7536599
Casilio Imaging EpEd Mammalian Expression Vector pmax-PUF9R_mRuby2 Kanamycin CMV Promoter RFP tag Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
PUF9R_mRuby2
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-PUF9R_mRuby2
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Fusion Tag:
RFP tag
Expression:
Transient
Catalog No. ABIN7536600
Casilio Imaging EpEd Mammalian Expression Vector pCR8-sgRNA-15xPBSc Spectinomycin T7 Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
SgRNA-15xPBSc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pCR8-sgRNA-15xPBSc
Resistance Gene:
Spectinomycin
Promoter:
T7 Promoter
Expression:
Transient
Catalog No. ABIN7536603
Casilio Imaging EpEd Mammalian Expression Vector pCR8-sgRNA-15xPBS9R Spectinomycin T7 Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
SgRNA-15xPBS9R
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pCR8-sgRNA-15xPBS9R
Resistance Gene:
Spectinomycin
Promoter:
T7 Promoter
Expression:
Transient
Catalog No. ABIN7536604
Casilio Imaging EpEd Mammalian Expression Vector pCR8-sgRNA-20xPBS48107 Spectinomycin T7 Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
SgRNA-20xPBS48107
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pCR8-sgRNA-20xPBS48107
Resistance Gene:
Spectinomycin
Promoter:
T7 Promoter
Expression:
Transient
Catalog No. ABIN7536605
Casilio Imaging EpEd Mammalian Expression Vector pCR8-sgRNA-25xPBS9R Spectinomycin T7 Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
SgRNA-25xPBS9R
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pCR8-sgRNA-25xPBS9R
Resistance Gene:
Spectinomycin
Promoter:
T7 Promoter
Expression:
Transient
Catalog No. ABIN7536607
Casilio Imaging EpEd Mammalian Expression Vector pCR8-sgRNA-20xPBSc Spectinomycin T7 Promoter unconjugated Transient
Type:
Casilio Imaging
Application:
Epigenetic Editing
Gene:
SgRNA-20xPBSc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pCR8-sgRNA-20xPBSc
Resistance Gene:
Spectinomycin
Promoter:
T7 Promoter
Expression:
Transient
Catalog No. ABIN7536606
  • Type
    • Plasmid
  • Supplier
    • antibodies-online
  • Target
    • Clover_PUF48107
    • Clover_PUFc
    • PUF9R_iRFP670
    • PUF9R_mRuby2
    • SgRNA-15xPBS9R
    • SgRNA-15xPBSc
    • SgRNA-20xPBS48107
    • SgRNA-20xPBSc
    • SgRNA-25xPBS9R
    • dCas9
    • iRFP670_PUFc
  • Reactivity
    • Various Species
  • Application
    • Epigenetic Editing (EpEd)
  • Conjugate
    • Un-conjugated
    • RFP tag
    • GFP tag
  • Vector Backbone
    • lenti_dCas9_Blast
    • pCR8-sgRNA-15xPBS9R
    • pCR8-sgRNA-15xPBSc
    • pCR8-sgRNA-20xPBS48107
    • pCR8-sgRNA-20xPBSc
    • pCR8-sgRNA-25xPBS9R
    • pmax-Clover_PUF48107
    • pmax-Clover_PUFc
    • pmax-PUF9R_iRFP670
    • pmax-PUF9R_mRuby2
    • pmax-iRFP670_PUFc
  • Vector
    • Mammalian Expression Vector
    • Lentiviral Vector
  • Insert Casilio Imaging
    • Casilio Imaging
    • cDNA
    • ORF
    • shRNA
    • gRNA + Cas9
    • gRNA
    • Empty
    • Casilio Regulation
    • Casilio HDR
  • Bacterial Resistance
    • Kanamycin
    • Spectinomycin
    • Ampicillin
  • Expression Type
    • Transient
  • Selectable Marker
    • Blasticidin
  • Promoter
    • CMV Promoter
    • T7 Promoter
    • EFS Promoter
  • Format
    • Liquid
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