(4 references)
Catalog No. ABIN3339939
Supplier Product No.: sr300143
$425.70
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 8 Business Days
Quick Overview for Human AKT1 siRNA Oligo (ABIN3339939)
Gene
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Species
Human
Application
RNA Interference (RNAi)
Purification
HPLC purified
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Supplier Product No.
- sr300143
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Supplier
- OriGene
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Oligo-Type
- siRNA Oligo
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Purpose
- siRNA (27 mer) kit with 3 gene-specific unique siRNA duplexes and negative control for gene knockdown.
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Brand
- Trilencer-27
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Sequence
- Available with shipment
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Components
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- AKT1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
- Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
- RNAse free siRNA Duplex Resuspension Buffer - 2 ml
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Application Notes
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- No. of transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)
- Quality Control: Tested by ESI-MS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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- 2 nmoles of each duplex is provided (including the control duplex). Addition of 100 μL of RNase-free Duplex Buffer will result in 20 μM final concentration, vortex thoroughly and microfuge prior to use.
- Heat to 94 °C for 2 minutes, remove from heat and allow tube to cool to room temperature. The oligos were dried in duplex form so heating may not be necessary, however following this protocol ensures that the contents will be fully duplexed.
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Storage
- -20 °C
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Storage Comment
- The dried duplexes can be stored at 4 °C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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Expiry Date
- 12 months
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: "Modulation of EZH2 Expression by MEK-ERK or PI3K-AKT Signaling in Lung Cancer Is Dictated by Different KRAS Oncogene Mutations." in: Cancer research, Vol. 76, Issue 3, pp. 675-85, (2016) (PubMed).
: "Concurrent Targeting of KRAS and AKT by MiR-4689 Is a Novel Treatment Against Mutant KRAS Colorectal Cancer." in: Molecular therapy. Nucleic acids, Vol. 4, pp. e231, (2015) (PubMed).
: "Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways." in: The Journal of biological chemistry, Vol. 289, Issue 1, pp. 510-9, (2014) (PubMed).
: "PI3K/AKT pathway mediates induction of IL-1RA by TSH in fibrocytes: modulation by PTEN." in: The Journal of clinical endocrinology and metabolism, Vol. 99, Issue 9, pp. 3363-72, (2014) (PubMed).
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: "Modulation of EZH2 Expression by MEK-ERK or PI3K-AKT Signaling in Lung Cancer Is Dictated by Different KRAS Oncogene Mutations." in: Cancer research, Vol. 76, Issue 3, pp. 675-85, (2016) (PubMed).
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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Alternative Name
- AKT1
Target
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