(1 reference)
Catalog No. ABIN3342047
Supplier Product No.: sr302265
$435.60
Plus shipping costs $50.00
1 kit
Shipping to:
United States
Delivery in 4 to 8 Business Days
Quick Overview for Human HSPD1 siRNA Oligo (ABIN3342047)
Gene
HSPD1
(Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
Species
Human
Application
RNA Interference (RNAi)
Purification
HPLC purified
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Supplier Product No.
- sr302265
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Supplier
- OriGene
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Oligo-Type
- siRNA Oligo
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Purpose
- siRNA (27 mer) kit with 3 gene-specific unique siRNA duplexes and negative control for gene knockdown.
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Brand
- Trilencer-27
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Sequence
- Available with shipment
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Components
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- HSPD1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
- Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
- RNAse free siRNA Duplex Resuspension Buffer - 2 ml
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Application Notes
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- No. of transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)
- Quality Control: Tested by ESI-MS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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- 2 nmoles of each duplex is provided (including the control duplex). Addition of 100 μL of RNase-free Duplex Buffer will result in 20 μM final concentration, vortex thoroughly and microfuge prior to use.
- Heat to 94 °C for 2 minutes, remove from heat and allow tube to cool to room temperature. The oligos were dried in duplex form so heating may not be necessary, however following this protocol ensures that the contents will be fully duplexed.
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Storage
- -20 °C
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Storage Comment
- The dried duplexes can be stored at 4 °C. However, once reconstituted with dH2O, the plasmids must be stored at -20°C.
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Expiry Date
- 12 months
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: "Lysine biotinylation and methionine oxidation in the heat shock protein HSP60 synergize in the elimination of reactive oxygen species in human cell cultures." in: The Journal of nutritional biochemistry, Vol. 25, Issue 4, pp. 475-82, (2014) (PubMed).
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: "Lysine biotinylation and methionine oxidation in the heat shock protein HSP60 synergize in the elimination of reactive oxygen species in human cell cultures." in: The Journal of nutritional biochemistry, Vol. 25, Issue 4, pp. 475-82, (2014) (PubMed).
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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Alternative Name
- HSPD1
Target
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