Primer and Probes

Amplification primers are short, single-stranded nucleotide sequences that are complementary to a fragment of the DNA or RNA that a researcher wants to amplify or sequence. Prior to the PCR amplification process, a primer hybridizes with its complementary sequence on a single strand of DNA or RNA. The formation of a short, double-stranded sequence permits binding and activity by a polymerase enzyme, kick-starting the amplification process.

Hybridization probes are short, labeled, single-stranded nucleotide sequences that are complementary to a fragment of DNA or RNA that the researcher wants to detect. Probes may be labeled with fluorescent dyes, or other chemicals that permit rapid, quantitative detection. Probes function by hybridizing with a desired complementary sequence, the desired sequence may then be detected via the label associated with the probe. We offer you a wide variety of primers and probes. if you have any questions regarding our products, please contact our customer service via live chat, email, or phone.