The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.
Protein expression can be induced using lactose or IPTG, but requires the use of DE3 prophage-positive E. coli expression strains. The resulting Spot-Tag® fusion protein can be purified using the ChromoTek Spot-Trap®.
Suitable host strains for propagation in E. coli include DH5alpha, HB101, XL1-Blue, and other general purpose strains. The vector confers resistance to kanamycin (50 μg/ml) to E. coli hosts. (This plasmid was designed and generated by Dr. Philipp Kaiser from the NMI.)

Vector Map
(Click image to enlarge)

Vector Map of pSpot2

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Multiple Cloning Site (Click image to enlarge)

Multiple cloning site image of pSpot2

Available Downloads

The following download material is available for vector pSpot2: