Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@genomics-online.com

pSpot4

The plasmid pSpot4 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot4, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Protein expression can be induced using lactose or IPTG, but requires the use of DE3 prophage-positive E. coli expression strains. The resulting Spot-Tag® fusion protein can be purified using the ChromoTek Spot-Trap®.
Suitable host strains for propagation in E. coli include DH5alpha, HB101, XL1-Blue, and other general purpose strains. The vector confers resistance to ampicillin (100 μg/ml) to E. coli hosts. (This plasmid was designed and generated by Dr. Philipp Kaiser from the NMI.)

Vector Map
(Click image to enlarge)

Search our products with this vector backbone
We offer a wide variety of inserts for this backbone. Search and compare our plasmid-based products.

Multiple Cloning Site (Click image to enlarge)

Available Downloads

The following download material is available for vector pSpot4:
You are here:
Support