pORF Vector is a Cloning Vector with Spectinomycin resistance that holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. The sequence of the Vector Map contains a 2443bp region between the AfIII and EcoRV cut site, which would be removed during the cloning process of the insert. AflII occurs at 554bp-560bp and EcoRV occurs at 2998bp-3003bp. The vector sequence contains the death gene that gets removed upon recombination with the ORF insert.
Vector Map (Click image to enlarge)
pORF Vector Map
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The following download material is available for vector pORF: