The pENTR223.1-LC is a gateway cloning vector for bacterial expression. The vector has a size of 3375 bp and contains the pBR322 origin of replication. This vector carries two genes, ccdB and CmR, in the region that will be replaced by cloned DNA segments. The ccdB gene (DNA gyrase inhibitor) provides strong negative selection against vector molecules retaining this region, whereas CmR (chloramphenicol resistance gene) provides positive selection for propagating the vector. Sequencing primer are T7, M13 and M13reverse. The pENTR223.1-LC is spectinomycin resistant up to 100 ug/ml.