pCMV6-XL4
Mammalien expression vector which contains a polylinker from Sac I to Sma I. The CMV promoter, which can be used to express the cloned cDNA, is followed by the hGH (human growth hormone) polyA signal located downstream of the insert. The ColE1 ori is the bacterial origin of replication, the SV40ori allows for replication in mammalian cells and the f1 ori is the filamentous phage origin of replication, which allows for the recovery of single-stranded plasmids. The ampicillin resistant gene confers the selection of the plasmid in E. coli. T7 RNA polymerase can be used for generating transcripts of the cDNA by in vitro transcription. However, the pCMVXL4 vector has a second but opposing T7 site (with a one-base substitution on the 3’ end) located between ColE1 ori and SV40 ori. Inserts (cDNA) in this vector must first be released by digesting with Sac I and Sma I before the in vitro transcription reaction.
Available Downloads
The following download material is available for vector pCMV6-XL4:Recommended next Filters:
4,799 results
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Serum Amyloid A 4 (SAA4)
- NCBI Accession:
- NM_006512,NP_006503
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Splicing Factor 3b, Subunit 5, 10kDa (SF3B5)
- NCBI Accession:
- NM_031287,NP_112577
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Secretory Leukocyte Peptidase Inhibitor (SLPI)
- NCBI Accession:
- NM_003064,NP_003055
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- TRAF Family Member-Associated NFKB Activator (TANK)
- NCBI Accession:
- NM_133484,NP_597841
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 2 (NUDT2)
- NCBI Accession:
- NM_147172,NP_671701
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 6 (NUDT6)
- NCBI Accession:
- NM_198041,NP_932158
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Outer Dense Fiber of Sperm Tails 2-Like (ODF2L)
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- O-Acyl-ADP-Ribose Deacylase 1 (OARD1)
- NCBI Accession:
- NM_145063,NP_659500
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Pterin-4 alpha-Carbinolamine Dehydratase/dimerization Cofactor of Hepatocyte Nuclear Factor 1 alpha (TCF1) 2 (PCBD2)
- NCBI Accession:
- NM_032151,NP_115527
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Ninein (GSK3B Interacting Protein) (NIN)
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- NOP10 Ribonucleoprotein Homolog (Yeast) (Nop10)
- NCBI Accession:
- NM_018648,NP_061118
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Neuronatin (NNAT)
- NCBI Accession:
- NM_005386,NP_005377
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nuclear Receptor 2C2-Associated Protein (NR2C2AP)
- NCBI Accession:
- NM_176880,NP_795361
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nuclear Transport Factor 2 (NUTF2)
- NCBI Accession:
- NM_005796,NP_005787
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- NTF2-Related Export Protein 1 (NXT1)
- NCBI Accession:
- NM_013248,NP_037380
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nuclear Protein, Transcriptional Regulator, 1 (NUPR1)
- NCBI Accession:
- NM_012385,NP_036517
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Oligosaccharyltransferase Complex Subunit (OSTC)
- NCBI Accession:
- NM_021227,NP_067050
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- P Antigen Family, Member 4 (Prostate Associated) (PAGE4)
- NCBI Accession:
- NM_007003,NP_008934
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Prefoldin Subunit 6 (PFDN6)
- NCBI Accession:
- NM_014260,NP_055075
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Profilin 2 (PFN2)
- NCBI Accession:
- NM_002628,NP_002619
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient