pCMV6-XL4
Mammalien expression vector which contains a polylinker from Sac I to Sma I. The CMV promoter, which can be used to express the cloned cDNA, is followed by the hGH (human growth hormone) polyA signal located downstream of the insert. The ColE1 ori is the bacterial origin of replication, the SV40ori allows for replication in mammalian cells and the f1 ori is the filamentous phage origin of replication, which allows for the recovery of single-stranded plasmids. The ampicillin resistant gene confers the selection of the plasmid in E. coli. T7 RNA polymerase can be used for generating transcripts of the cDNA by in vitro transcription. However, the pCMVXL4 vector has a second but opposing T7 site (with a one-base substitution on the 3’ end) located between ColE1 ori and SV40 ori. Inserts (cDNA) in this vector must first be released by digesting with Sac I and Sma I before the in vitro transcription reaction.
Available Downloads
The following download material is available for vector pCMV6-XL4:Recommended next Filters:
4,799 results
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Integrin beta-8 (ITGB8)
- NCBI Accession:
- NM_002214,NP_002205
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Inter-alpha Trypsin Inhibitor, Heavy Chain 1 (ITIH1)
- NCBI Accession:
- NM_002215,NP_002206
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Integrin beta 3 (ITGB3)
- NCBI Accession:
- NM_000212,NP_000203
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Integrin alpha V (ITGAV)
- NCBI Accession:
- NM_002210,NP_002201
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- IL2-Inducible T-Cell Kinase (ITK)
- NCBI Accession:
- NM_005546,NP_005537
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Integrin alpha-L (ITGAL)
- NCBI Accession:
- NM_002209,NP_002200
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Inter-alpha Globulin Inhibitor H2 Polypeptide (ITIH2)
- NCBI Accession:
- NM_002216,NP_002207
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- IWS1 Homolog (IWS1)
- NCBI Accession:
- NM_017969,NP_060439
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Integrin beta-6 (ITGB6)
- NCBI Accession:
- NM_000888,NP_000879
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Kallmann Syndrome 1 Sequence (KAL1)
- NCBI Accession:
- NM_000216,NP_000207
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- KN Motif and Ankyrin Repeat Domains 3 (KANK3)
- NCBI Accession:
- NM_198471,NP_940873
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Katanin P60 Subunit A-Like 1 (KATNAL1)
- NCBI Accession:
- NM_032116,NP_115492
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Kelch Repeat and BTB (POZ) Domain Containing 6 (KBTBD6)
- NCBI Accession:
- NM_152903,NP_690867
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 3 (KCNA3)
- NCBI Accession:
- NM_002232,NP_002223
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Ankyrin Repeat Domain 15 (ANKRD15)
- NCBI Accession:
- NM_153186,NP_694856
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Kazal-Type Serine Peptidase Inhibitor Domain 1 (Kazald1)
- NCBI Accession:
- NM_030929,NP_112191
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 5 (KCNA5)
- NCBI Accession:
- NM_002234,NP_002225
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Shaker-Related Subfamily, beta Member 2 (KCNAB2)
- NCBI Accession:
- NM_172130,NP_742128
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 1 (KCNB1)
- NCBI Accession:
- NM_004975,NP_004966
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Shaw-Related Subfamily, Member 2 (KCNC2)
- NCBI Accession:
- NM_153748,NP_715624
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient