pBluescript II SK+
The pBluescript II SK+ phagemid (plasmids with a phage origin) is a cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II SK+ phagemid has an extensive polylinker with 21 unique restriction enzyme recognition sites. It is a high copy number ColE1-based phagemid with a large and versatile polylinker Sac-->Kpn; f1 origin in (+) orientation. The T3 and T7 promoters are for in vitro transcription of RNA.