The pBluescript II SK+ phagemid (plasmids with a phage origin) is a cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II SK+ phagemid has an extensive polylinker with 21 unique restriction enzyme recognition sites. It is a high copy number ColE1-based phagemid with a large and versatile polylinker Sac-->Kpn; f1 origin in (+) orientation. The T3 and T7 promoters are for in vitro transcription of RNA.
Vector Map (Click image to enlarge)
pBluescript II SK+ Vector Map
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The following download material is available for vector pBluescript II SK+: