Mammalian vector with non-tagged expression: This vector is essentially the same as the pCMV6-Entry vector, except for the absence of the C-terminal Myc-DDK tag.
ORF cloned into this vector at SgfI and RsrII sites will express the ORF-encoded protein plus two additional amino acids at the C-terminus, T and R.
The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.