5-hmC has been found to be elevated in certain mammalian tissues, such human and mouse brain, as well as in embryonic stem cells. It is thought to determine developmental fates and, when present in aberrant patterns, contribute to disease.
A complete understanding of the potential function 5-hmC requires an accurate, scalable sequencing method.
Traditional bisulfite sequencing or methylated CpG island recovery assay (MIRA) cannot distinguish between 5-methylcytosine (5-mC), the most common and widely studied DNA-methylation, and 5-hmC.
TAB-seq (Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine) is a modification of the standard bisulfite sequencing method through which 5-hydroxymethylcytosine (5-hmC) can be specifically identified. In conjunction with the traditional methods TAB-seq allows mapping of both methylation variants at single-base resolution either on a genomic scale or for a particular locus.
TAB-seq was first introduced in 2012. Until now it has been used to study the role of 5hmC in brain functions, cancers and cognitive several cognitive disorders.
genomics-online now offers TAB-seq kits and DNA standards for TAB-seq. Please check out our brand new TAB-seq products or get into contact with us via Chat, phone or email for more information.
Our TAB-seq related products:
|Product Name||Product Id||Application||Description|
|5hmC DNA Standard||ABIN1741996||TAB-Seq||5-Hydroxymethylated pUC19 DNA as 5hmC spike-in control for ABIN1741992 and ABIN1741993|
|5mC DNA Standard||ABIN1741995||TAB-Seq||Lambda DNA with methylation of all cytosines at CpG sites as 5mC/C spike-in control for TAB-Seq (ABIN1741992) or other applicaitons (ABIN1741995.)|
|5mC Tet1 Oxidation Kit||ABIN1741993||TAB-Seq||5mC Tet1 Oxidation Kit can be used to convert 5-methylcytosine (5mC) in genomic DNA to 5-carboxylcytosine (5caC) for subsequent sequencing applications. Each kit contains enough reagents for up to 3 genome-wide or 9 loci-specific sequencing reactions based on recommended protocols.|
|5mC Tet1 Oxidation Kit||ABIN1741994||TAB-Seq||This 5mC Tet1 Oxidation Kit is specifically designed to be used to convert 5-methylcytosine (5mC) in genomic DNA to 5-carboxylcytosine (5caC) for subsequent sequencing applications on the PacBio® RS instrument.Each kit contains enough reagents for up to 3 genome-wide or 9 loci-specific sequencing reactions based on recommended protocols.|
|5hmC TAB-Seq Kit||ABIN1741992||TAB-Seq||The 5hmC TAB-Seq Kit can be used to prepare genomic DNA to differentiate 5-hydroxylmethylcytosine (5hmC) from 5-methylcytosine (5mC) for subsequent sequencing applications by converting 5mC to 5-carboxylcytosine (5caC) while keeping 5hmC protected. Each kit contains enough reagents for up to 3 genome-wide or 9 loci-specific sequencing reactions based on recommended protocols.|
Revealing the unknown - TAB-seq reveals 5hmC DNA modifications
Bisulfite sequencing alone cannot distinguish 5mC and 5hmC. The combination with TAB-seq reveals locations of both cytosine modifications. Our TAB-seq kits and reagents will help you.
genomics-online now offers TAB-seq kits and DNA standards for TAB-seq. Please check out our brand new TAB-seq products. Click here
Additional reading about TAB-seq method:
- Yu, M., Hon, G.C., Szulwach, K.E., Song, C.X., Zhang, L., Kim, A., Li, X., Dai, Q., Shen, Y., Park, B., et al. (2012). Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome. Cell 149, 1368-1380.
- Mariani, C.J., Madzo, J., Moen, E.L., Yesilkanal, A., and Godley, L.A. (2013). Alterations of 5-hydroxymethylcytosine in human cancers. Cancers (Basel) 5, 786-814.
- Chopra, P., Papale, L.A., White, A.T., Hatch, A., Brown, R.M., Garthwaite, M.A., Roseboom, P.H., Golos, T.G., Warren, S.T., and Alisch, R.S. (2014). Array-based assay detects genome-wide 5-mC and 5-hmC in the brains of humans, non-human primates, and mice. BMC Genomics 15, 131-2164-15-131.