qPCR Primer

Quantitative PCR (qPCR/rtPCR) is a special form of polymerase chain reaction that uses labeled nucleotides to quantify the number of copies of a specific DNA or RNA sequence in a mixture. A qPCR reaction is carried out much like a traditional PCR reaction. A primer hybridizes with a complementary sequence on a single-stranded target nucleotide string, and a polymerase enzyme gathers nucleotides from the surrounding mixture and extends the complementary chain.