pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Lentiviral CRISPR vector encoding a guide RNA (gRNA), plus Cas9 and a puromycin resistance marker. The Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. The gRNA is under the control of the U6 promoter.
Available Downloads
The following download material is available for vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro:Recommended next Filters:
115,399 results
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1)
- NCBI Accession:
- NM_145813
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1)
- NCBI Accession:
- NM_031356
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- F-Box Protein 3 (FBXO3)
- NCBI Accession:
- NM_033406
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- F-Box Protein 3 (FBXO3)
- NCBI Accession:
- NM_001109606
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- F-Box Protein 3 (FBXO3)
- NCBI Accession:
- NM_020593
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR)
- NCBI Accession:
- NM_021050
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR)
- NCBI Accession:
- NM_000492
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Acetoacetyl-CoA Synthetase (AACS)
- NCBI Accession:
- NM_023104
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Acetoacetyl-CoA Synthetase (AACS)
- NCBI Accession:
- NM_030210
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Acetoacetyl-CoA Synthetase (AACS)
- NCBI Accession:
- NM_023928
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT)
- NCBI Accession:
- NM_001106798
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT)
- NCBI Accession:
- NM_026276
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT)
- NCBI Accession:
- NM_015423
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B)
- NCBI Accession:
- NM_033622
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Brain and Acute Leukemia, Cytoplasmic (BAALC)
- NCBI Accession:
- NM_080640
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Brain and Acute Leukemia, Cytoplasmic (BAALC)
- NCBI Accession:
- NM_024812
- Species:
- Human
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Brain and Acute Leukemia, Cytoplasmic (BAALC)
- NCBI Accession:
- NM_144762
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Aspartyl-tRNA Synthetase (DARS)
- NCBI Accession:
- NM_053799
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Aspartyl-tRNA Synthetase (DARS)
- NCBI Accession:
- NM_145507
- Species:
- Mouse
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient
- Type:
- gRNA + Cas9
- Application:
- Protein Expression, Genome Editing with Engineered Nucleases
- Gene:
- Cofactor of BRCA1 (COBRA1)
- NCBI Accession:
- NM_001107817
- Species:
- Rat
- Vector type:
- Lentiviral Vector
- Vector backbone:
- pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
- Resistance Gene:
- Ampicillin
- Selection Marker:
- Puromycin
- Promoter:
- U6 Promoter, SFFV Promoter
- Expression:
- Stable, Transient