pCMV6-XL4
Mammalien expression vector which contains a polylinker from Sac I to Sma I. The CMV promoter, which can be used to express the cloned cDNA, is followed by the hGH (human growth hormone) polyA signal located downstream of the insert. The ColE1 ori is the bacterial origin of replication, the SV40ori allows for replication in mammalian cells and the f1 ori is the filamentous phage origin of replication, which allows for the recovery of single-stranded plasmids. The ampicillin resistant gene confers the selection of the plasmid in E. coli. T7 RNA polymerase can be used for generating transcripts of the cDNA by in vitro transcription. However, the pCMVXL4 vector has a second but opposing T7 site (with a one-base substitution on the 3’ end) located between ColE1 ori and SV40 ori. Inserts (cDNA) in this vector must first be released by digesting with Sac I and Sma I before the in vitro transcription reaction.
Available Downloads
The following download material is available for vector pCMV6-XL4:Recommended next Filters:
4,799 results
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- HERV-H LTR-Associating 3 (HHLA3)
- NCBI Accession:
- NM_007071,NP_009002
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Islet Cell Autoantigen 1,69kDa-Like (ICA1L)
- NCBI Accession:
- NM_178231,NP_839945
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4)
- NCBI Accession:
- NM_001546,NP_001537
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Inhibitor of KB Kinase beta Interacting Protein (IKbIP)
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Interleukin 1 delta (FIL1d)
- NCBI Accession:
- NM_012275,NP_036407
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Jun Dimerization Protein 2 (JDP2)
- NCBI Accession:
- NM_130469,NP_569736
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- HIG1 Hypoxia Inducible Domain Family, Member 2A (HIGD2A)
- NCBI Accession:
- NM_138820,NP_620175
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Histone Cluster 1, H2bg (HIST1H2BG)
- NCBI Accession:
- NM_003518,NP_003509
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Histone Cluster 1, H2al (HIST1H2AL)
- NCBI Accession:
- NM_003511,NP_003502
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- High Mobility Group Nucleosome Binding Domain 1 (HMGN1)
- NCBI Accession:
- NM_004965,NP_004956
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Heat Shock 70kDa Protein 4 (HSPA4)
- NCBI Accession:
- NM_198431,NP_940833
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Heat Shock Protein Family B (Small), Member 11 (HSPB11)
- NCBI Accession:
- NM_016126,NP_057210
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Iron-Sulfur Cluster Scaffold Homolog (E. Coli) (ISCU)
- NCBI Accession:
- NM_014301,NP_055116
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Eukaryotic Translation Initiation Factor 6 (EIF6)
- NCBI Accession:
- NM_181467,NP_852132
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Potassium Voltage-Gated Channel, Isk-Related Family, Member 3 (Kcne3)
- NCBI Accession:
- NM_005472,NP_005463
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- PCNA Clamp Associated Factor (PAF)
- NCBI Accession:
- NM_014736,NP_055551
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Keratinocyte Associated Protein 2 (KRTCAP2)
- NCBI Accession:
- NM_173852,NP_776251
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Lectin, Galactoside-Binding, Soluble, 2 (LGALS2)
- NCBI Accession:
- NM_006498,NP_006489
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Lectin, Galactoside-Binding, Soluble, 14 (LGALS14)
- NCBI Accession:
- NM_020129,NP_064514
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- LIM Domain Containing 2 (LIMD2)
- NCBI Accession:
- NM_030576,NP_085053
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient