pCMV6-XL4
Mammalien expression vector which contains a polylinker from Sac I to Sma I. The CMV promoter, which can be used to express the cloned cDNA, is followed by the hGH (human growth hormone) polyA signal located downstream of the insert. The ColE1 ori is the bacterial origin of replication, the SV40ori allows for replication in mammalian cells and the f1 ori is the filamentous phage origin of replication, which allows for the recovery of single-stranded plasmids. The ampicillin resistant gene confers the selection of the plasmid in E. coli. T7 RNA polymerase can be used for generating transcripts of the cDNA by in vitro transcription. However, the pCMVXL4 vector has a second but opposing T7 site (with a one-base substitution on the 3’ end) located between ColE1 ori and SV40 ori. Inserts (cDNA) in this vector must first be released by digesting with Sac I and Sma I before the in vitro transcription reaction.
Available Downloads
The following download material is available for vector pCMV6-XL4:Recommended next Filters:
4,799 results
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3)
- NCBI Accession:
- NM_012396,NP_036528
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Phosphatidylinositol Glycan Anchor Biosynthesis, Class P (PIGP)
- NCBI Accession:
- NM_153682,NP_710149
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A)
- NCBI Accession:
- NM_000300,NP_000291
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB)
- NCBI Accession:
- NM_181795,NP_861460
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor alpha (PKIA)
- NCBI Accession:
- NM_006823,NP_006814
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Placenta-Specific 4 (PLAC4)
- NCBI Accession:
- NM_182832,NP_878252
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Plasminogen-Like B2 (PLGLB2)
- NCBI Accession:
- NM_002665,NP_002656
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Phospholamban (PLN)
- NCBI Accession:
- NM_002667,NP_002658
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I)
- NCBI Accession:
- NM_006233,NP_006224
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4)
- NCBI Accession:
- NM_021173,NP_066996
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP)
- NCBI Accession:
- NM_002704,NP_002695
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- G Substrate (Gsbs)
- NCBI Accession:
- NM_006658,NP_006649
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14B (PPP1R14B)
- NCBI Accession:
- NM_138689,NP_619634
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14C (PPP1R14C)
- NCBI Accession:
- NM_030949,NP_112211
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- StAR-Related Lipid Transfer (START) Domain Containing 5 (STARD5)
- NCBI Accession:
- NM_030574,NP_085051
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2)
- NCBI Accession:
- NM_207013,NP_996896
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B) (TCEB2)
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Transcription Elongation Factor A (SII)-Like 8 (TCEAL8)
- NCBI Accession:
- NM_153333,NP_699164
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Nectin cell adhesion molecule 3 (NECTIN3)
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Ribosomal Protein L32 (RPL32)
- NCBI Accession:
- NM_000994,NP_000985
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient