pCMV6-XL4
Mammalien expression vector which contains a polylinker from Sac I to Sma I. The CMV promoter, which can be used to express the cloned cDNA, is followed by the hGH (human growth hormone) polyA signal located downstream of the insert. The ColE1 ori is the bacterial origin of replication, the SV40ori allows for replication in mammalian cells and the f1 ori is the filamentous phage origin of replication, which allows for the recovery of single-stranded plasmids. The ampicillin resistant gene confers the selection of the plasmid in E. coli. T7 RNA polymerase can be used for generating transcripts of the cDNA by in vitro transcription. However, the pCMVXL4 vector has a second but opposing T7 site (with a one-base substitution on the 3’ end) located between ColE1 ori and SV40 ori. Inserts (cDNA) in this vector must first be released by digesting with Sac I and Sma I before the in vitro transcription reaction.
Available Downloads
The following download material is available for vector pCMV6-XL4:Recommended next Filters:
4,799 results
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Melanoma Inhibitory Activity (MIA)
- NCBI Accession:
- NM_006533,NP_006524
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- LSM5 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM5)
- NCBI Accession:
- NM_012322,NP_036454
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- LSM7 Homolog, U6 Small Nuclear RNA Associated (S. Cerevisiae) (LSM7)
- NCBI Accession:
- NM_016199,NP_057283
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Lymphocyte Antigen 6 Complex, Locus H (LY6H)
- NCBI Accession:
- NM_002347,NP_002338
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Family, Protein K (Avian) (MAFK)
- NCBI Accession:
- NM_002360,NP_002351
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Transcription Factor MafG (Mafg)
- NCBI Accession:
- NM_002359,NP_002350
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Multiple Coagulation Factor Deficiency 2 (MCFD2)
- NCBI Accession:
- NM_139279,NP_644808
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Membrane Magnesium Transporter 1 (MMGT1)
- NCBI Accession:
- NM_173470,NP_775741
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- MORN Repeat Containing 4 (MORN4)
- NCBI Accession:
- NM_178832,NP_849154
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- MORN Repeat Containing 2 (MORN2)
- NCBI Accession:
- NM_194270,NP_919246
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Brain Protein 44-Like (BRP44L)
- NCBI Accession:
- NM_016098,NP_057182
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L20 (MRPL20)
- NCBI Accession:
- NM_017971,NP_060441
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L33 (MRPL33)
- NCBI Accession:
- NM_145330,NP_663303
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L30 (MRPL30)
- NCBI Accession:
- NM_145212,NP_660213
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L30 (MRPL30)
- NCBI Accession:
- NM_145213,NP_660214
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L36 (MRPL36)
- NCBI Accession:
- NM_032479,NP_115868
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L34 (MRPL34)
- NCBI Accession:
- NM_023937,NP_076426
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L41 (MRPL41)
- NCBI Accession:
- NM_032477,NP_115866
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L50 (MRPL50)
- NCBI Accession:
- NM_019051,NP_061924
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient
- Type:
- cDNA
- Application:
- Protein Expression
- Gene:
- Mitochondrial Ribosomal Protein L42 (MRPL42)
- NCBI Accession:
- NM_172177,NP_751917
- Species:
- Human
- Vector type:
- Mammalian Expression Vector
- Vector backbone:
- pCMV6-XL4
- Resistance Gene:
- Ampicillin
- Promoter:
- Enhanced CMV Promoter, T7 Promoter
- Expression:
- Transient