Bacterial expression vector with dual tag: Protein expression will be driven by a T7 promoter, with a 6x Histidine Tag followed by Glutathione-S-Transferase at the N terminal. Inserts are flanked by and can be excised using NheI and XhoI as long as inserts do not contain any internal NheI or XhoI sites. The His-GST Tag is cleavable with TEV.
Vector Map (Click image to enlarge)
pPB-His-GST Vector Map
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The following download material is available for vector pPB-His-GST: