Bacterial expression vector with single tag: Protein expression will be driven by a T7 promoter, with a His Tag at the N terminal. Inserts are flanked by and can be excised using NheI and XhoI as long as inserts do not contain any internal NheI or XhoI sites. The His Tag is cleavable with Thrombin.
Vector Map (Click image to enlarge)
pPB-N-His Vector Map
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The following download material is available for vector pPB-N-His: