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Safe-Red™

SDS, AGE
Pubmed (1 reference)
Catalog No. ABIN4219139
$66.32
Plus shipping costs $45.00
1 mL
Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
    Purpose
    Safe-Red™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
    Brand
    SafeView™
    Specificity
    UV Compatible
    Not Blue Light Compatible
    Sensitivity limit: 0.3-0.8 ng DNA per band
    Characteristics
    Convenient: Safe-Red™ is provided as a 6X loading dye, and is mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress.
    Easy to Use: View and document your results as you would with EtBr staining. Safe-Red™ can be excited with UV light, and has maximum emission at 630 nm.
    Safe: Non-carcinogenic.
    Sensitive: Detect as little as 0.3 - 0.8 ng of DNA per gel band.
    Superior: EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Red™ minimizes such damage, yielding higher transformation rates and lower mutation rates verses EtBr.
  • Application Notes
    Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
    Comment

    1. Prepare a 100 ml agarose or polyacrylamide solution.
    2. Mix gently without introducing any air bubbles.
    3. For agarose gel, let the solution cool down to 60 - 70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
    4. Mix samples and DNA marker with SafeViewTM dye at a 1:5 (dye : sample) dilution rate.
    5. Following electrophoresis, view the results under UV.

    Restrictions
    For Research Use only
  • Handling Advice
    Dispose Safe-Red™ as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
    Storage
    4 °C
    Storage Comment
    Store at 4°C for up to 2 years. Ships on blue ice.
  • Vlasschaert, Xia, Gray: "Selection preserves Ubiquitin Specific Protease 4 alternative exon skipping in therian mammals." in: Scientific reports, Vol. 6, pp. 20039, (2016) (PubMed).

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