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SafeView™ Classic

SDS, AGE
Pubmed (8 references)
Catalog No. ABIN4219136
$66.32
Plus shipping costs $45.00
1 mL
Shipping to: United States
Delivery in 11 to 21 Business Days
  • Application
    SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
    Purpose
    SafeView™ Classic is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
    Brand
    SafeView™
    Specificity
    UV Compatible
    Blue Light Compatible
    Sensitivity limit: 0.1-0.3 ng DNA per band
    Characteristics
    Easy to Use: SafeView™ Classic directly replaces EtBr. View and document your results as you would with EtBr staining. SafeView™ Classic can be viewed under UV light or blue light.
    Safe: Non-carcinogenic.
    Sensitive: Detect as little as 0.1 - 0.3 ng of DNA per gel band.
    Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    1. Prepare a 100 ml agarose or polyacrylamide solution.
    2. Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.
    3. Mix gently, the solution should have no air bubbles.
    4. For agarose gel, let the solution cool down to 60-70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
    5. Run gel electrophoresis with 5 μl SafeViewTM Classic / SafeViewTM FireRed per 100 ml buffer.
    6. View the results under UV or blue LED light.

    Restrictions
    For Research Use only
  • Handling Advice
    Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
    Storage
    4 °C
    Storage Comment
    Store at 4°C for up to 2 years. Ships on blue ice.
  • Doh, Kim, Oh, Lee: "Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker." in: Genes & genomics, Vol. 39, Issue 1, pp. 101-109, (2017) (PubMed).

    Dudek, Gossan, Yang, Im, Ruckshanthi, Yoshitane, Li, Jin, Wang, Boudiffa, Bellantuono, Fukada, Boot-Handford, Meng: "The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 365-76, (2016) (PubMed).

    Do, Kim, Yang, Pyo, Kim, Lee, Do, Kim, Chae, Sohn: "Decreased expression of p27 is associated with malignant transformation and extrathyroidal extension in papillary thyroid carcinoma." in: Tumour biology, Vol. 37, Issue 3, pp. 3359-64, (2016) (PubMed).

    Zhao, Tsao, Lee, Tsai, Wang, Chuang, Wu, Lin: "Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies." in: Journal of virological methods, Vol. 233, pp. 15-22, (2016) (PubMed).

    Zu, Tan, Ren, Ying: "Facile and phase-defined determination of HLA alleles with morpholino-functionalized nanoparticle probes." in: Nanomedicine : nanotechnology, biology, and medicine, (2016) (PubMed).

    Mizan, Jahid, Kim, Lee, Kim, Ha et al.: "Variability in biofilm formation correlates with hydrophobicity and quorum sensing among Vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in ..." in: Biofouling, Vol. 32, Issue 4, pp. 497-509, (2016) (PubMed).

    Vivero, Jaramillo, Cadavid-Restrepo, Soto, Herrera: "Structural differences in gut bacteria communities in developmental stages of natural populations of Lutzomyia evansi from Colombia's Caribbean coast." in: Parasites & vectors, Vol. 9, pp. 496, (2016) (PubMed).

    Anjali, Ganga, Nadiya, Shefeek, Sabu: "Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis." in: SpringerPlus, Vol. 5, Issue 1, pp. 1560, (2016) (PubMed).

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