(8 references)-
- Application
- SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
- Purpose
- SafeView™ Classic is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
- Brand
- SafeView™
- Specificity
-
UV Compatible
Blue Light Compatible
Sensitivity limit: 0.1-0.3 ng DNA per band - Characteristics
-
Easy to Use: SafeView™ Classic directly replaces EtBr. View and document your results as you would with EtBr staining. SafeView™ Classic can be viewed under UV light or blue light.
Safe: Non-carcinogenic.
Sensitive: Detect as little as 0.1 - 0.3 ng of DNA per gel band.
Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
1. Prepare a 100 ml agarose or polyacrylamide solution.
2. Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.
3. Mix gently, the solution should have no air bubbles.
4. For agarose gel, let the solution cool down to 60-70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
5. Run gel electrophoresis with 5 μl SafeViewTM Classic / SafeViewTM FireRed per 100 ml buffer.
6. View the results under UV or blue LED light. - Restrictions
- For Research Use only
-
- Handling Advice
- Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
- Storage
- 4 °C
- Storage Comment
- Store at 4°C for up to 2 years. Ships on blue ice.
-
-
: "Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker." in: Genes & genomics, Vol. 39, Issue 1, pp. 101-109, (2017) (PubMed).
: "The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 365-76, (2016) (PubMed).
: "Decreased expression of p27 is associated with malignant transformation and extrathyroidal extension in papillary thyroid carcinoma." in: Tumour biology, Vol. 37, Issue 3, pp. 3359-64, (2016) (PubMed).
: "Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies." in: Journal of virological methods, Vol. 233, pp. 15-22, (2016) (PubMed).
: "Facile and phase-defined determination of HLA alleles with morpholino-functionalized nanoparticle probes." in: Nanomedicine : nanotechnology, biology, and medicine, (2016) (PubMed).
: "Variability in biofilm formation correlates with hydrophobicity and quorum sensing among Vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in ..." in: Biofouling, Vol. 32, Issue 4, pp. 497-509, (2016) (PubMed).
: "Structural differences in gut bacteria communities in developmental stages of natural populations of Lutzomyia evansi from Colombia's Caribbean coast." in: Parasites & vectors, Vol. 9, pp. 496, (2016) (PubMed).
: "Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis." in: SpringerPlus, Vol. 5, Issue 1, pp. 1560, (2016) (PubMed).
-
: "Identification and monitoring of Korean medicines derived from Cinnamomum spp. by using ITS and DNA marker." in: Genes & genomics, Vol. 39, Issue 1, pp. 101-109, (2017) (PubMed).
-
You are here:
