SafeView™ Classic

Details for Product No. ABIN4219136,
SDS-PAGE (SDS), Agarose Gel Electrophoresis (AGE)
Purpose SafeView™ Classic is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.
Brand SafeView™
Specificity UV Compatible
Blue Light Compatible
Sensitivity limit: 0.1-0.3 ng DNA per band
Characteristics Easy to Use: SafeView™ Classic directly replaces EtBr. View and document your results as you would with EtBr staining. SafeView™ Classic can be viewed under UV light or blue light.
Safe: Non-carcinogenic.
Sensitive: Detect as little as 0.1 - 0.3 ng of DNA per gel band.
Unique: SafeView™ Classic is the only nucleic acid stain that can differentiate DNA and RNA in gels, by staining DNA green (max emission 520 nm) and RNA red (max emission 635 nm).
Application Notes Optimal working dilution should be determined by the investigator.

1. Prepare a 100 ml agarose or polyacrylamide solution.
2. Add 5 μl SafeViewTM Classic / SafeViewTM FireRed to the gel solution.
3. Mix gently, the solution should have no air bubbles.
4. For agarose gel, let the solution cool down to 60-70°C and cast the gel. For polyacrylamide gel, add APS and TEMED and cast the gel according to regular polyacrylamide gel casting protocol.
5. Run gel electrophoresis with 5 μl SafeViewTM Classic / SafeViewTM FireRed per 100 ml buffer.
6. View the results under UV or blue LED light.

Restrictions For Research Use only
Handling Advice Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange, Propidium iodide).
Storage 4 °C
Storage Comment Store at 4°C for up to 2 years. Ships on blue ice.
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Zu, Tan, Ren, Ying: "Facile and phase-defined determination of HLA alleles with morpholino-functionalized nanoparticle probes." in: Nanomedicine : nanotechnology, biology, and medicine, 2016 (PubMed).

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Anjali, Ganga, Nadiya, Shefeek, Sabu: "Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis." in: SpringerPlus, Vol. 5, Issue 1, pp. 1560, 2016 (PubMed).