DNA dA-Tailing Kit

Product Details (hide)

Application

DNA Modification (DNA Mod)

Purpose

The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3' end of a blunt-ended DNA fragment.

Characteristics

This incorporated 3'-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3'-dT overhangs. The Klenow Fragment (3´→ 5´ Exo-) in this kit adds the 3'-dA via its DNA polymerase activity. This enzyme lacks 5'→3' exonuclease activity and has mutations that effectively abolish the inherent 3'→5' exonuclease activity, thus preventing degradation of this 3'-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3´→ 5´ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038).

Unit Definition

One unit is defined as the amount of Klenow Fragment (3'→5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.

Alternatives (show)

Application Details (hide)

Comment

• DNA sample preparation
• dATailing of 1 5 μg of blunt ended DNA

Restrictions

For Research Use only

Handling (hide)

Buffer

25 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.

Storage

-20 °C

Storage Comment

Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly.