Tris-Borate-EDTA TBE Buffer (10X)
AGE
10 X
Catalog No. ABIN925559
Supplier Product No.: mb-019
Quick Overview for Tris-Borate-EDTA TBE Buffer (10X) (ABIN925559)
Application
Agarose Gel Electrophoresis (AGE)
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Supplier Product No.
- mb-019
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Supplier
- Rockland
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Characteristics
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TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.
Synonyms: buffer containing boric acid and EDTA
Sterilization: Sterile Aseptic Filter
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Application Notes
- This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 X
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Storage
- RT
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