EcoRI ClearCut
RD
20000 U/mL
Catalog No. ABIN3188194
Quick Overview for EcoRI ClearCut (ABIN3188194)
Application
Restriction Digest (RD)
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Characteristics
- ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
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Components
- EcoRI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
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Comment
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- Restriction enzyme digestion of doublestranded DNA ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 515 minutes).
- Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
- Source: An E. coli strain that carries the modified EcoRI gene from E. coli RY13. The EcoRI enzyme recognizes and cuts the sequence G^AATTC.
- Reaction Buffer: Buffer 1: 10%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
- Heat Inactivation:65°C for 20 min
- Methylation Sensitivity:
- dam methylation: Not Sensitive
- dcm methylation: Not Sensitive
- CpG Methylation: Blocked by Some Combinations of Overlapping
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Restrictions
- For Research Use only
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Concentration
- 20000 U/mL
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Storage
- -20 °C
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