Exonuclease I, E. coli
DNA Mod
20 U/μL
Catalog No. ABIN3188195
Quick Overview for Exonuclease I, E. coli (ABIN3188195)
Application
DNA Modification (DNA Mod)
-
-
Characteristics
- Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3'→5' direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3'-terminus is phosphorylated.
-
Components
- Enzyme supplied with 10X Reaction Buffer
-
Unit Definition
- One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer.
-
-
-
-
Comment
-
- Removal of singlestranded primer oligonucleotides from:
- PCR mixtures for applications involving sequencing or labelling
- from nucleic acid mixtures
- Assaying for the presence of singlestranded DNA with a 3'hydroxyl terminus
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 20 U/μL
-
Buffer
- 20 mM HEPES ( pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50 % (v/v) Glycerol.
-
Storage
- -20 °C
-
-
You are here:
