NotI ClearCut
RD
20000 U/mL
Catalog No. ABIN3188202
Quick Overview for NotI ClearCut (ABIN3188202)
Application
Restriction Digest (RD)
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Characteristics
- ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
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Components
- NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
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Comment
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- Restriction enzyme digestion of doublestranded DNA
- Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
- Source: An E. coli strain that carries the modified NotI gene from Nocardia otitidiscaviarum (ATCC 14630). The NotI ClearCut enzyme recognizes and cuts the sequence GC^GGCCGC.
- Reaction Buffer: Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100%
- Methylation Sensitivity
- dam methylation: Not Sensitive
- dcm methylation: Not Sensitive
- CpG Methylation: Blocked
- Heat Inactivation: 65°C for 20 min
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Restrictions
- For Research Use only
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Concentration
- 20000 U/mL
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Buffer
- 10 mM Tris-HCl ( pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15 % Triton® X-100, and 50 % (v/v) Glycerol
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Storage
- -20 °C
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