RNase III, E. coli
RNA Mod
1 U/μL
Catalog No. ABIN3188207
Quick Overview for RNase III, E. coli (ABIN3188207)
Application
RNA Modification (RNA Mod)
-
-
Characteristics
- RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
-
Components
- Enzyme supplied with 10X Reaction Buffer
-
Unit Definition
- One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
-
-
-
-
Comment
-
- dsRNA digestion to short dsRNA fragments
- Useful for RNA processing, maturation and structure studies
- Directed cleavage of RNA
- siRNA preparation for RNA interference in mammalian cells
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 1 U/μL
-
Buffer
- 50 mM Tris-HCl ( pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
-
Storage
- -20 °C
-
-
You are here:
