2xTransTaq® High Fidelity(HiFi)PCR SuperMix II (-dye)
PCR
Quick Overview for 2xTransTaq® High Fidelity(HiFi)PCR SuperMix II (-dye) (ABIN5519416)
Application
Polymerase Chain Reaction (PCR)
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Purpose
- ready-to-use mixture for the amplification of genomic DNA
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Brand
- TransTaq®
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Specificity
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TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Extension rate is about 1-2 kb/min.
Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
Amplification of genomic DNA fragment up to 15 kb. -
Characteristics
- TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is provided at 2x concentration and can be used at 1x concentration by adding template, primers and H2O.
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Components
- 2xTransTaq® High Fidelity(HiFi)PCR SuperMix I (+dye ), ddH2O
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Application Notes
- Complex templates, GC/AT rich templates, Long PCR, High yield PCR
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Restrictions
- For Research Use only
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Storage
- -20 °C
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Storage Comment
- at -20°C for two years
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Expiry Date
- 24 months
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