EasyScript® Reverse Transcriptase
cDNA
Quick Overview for EasyScript® Reverse Transcriptase (ABIN5519431)
Application
cDNA Synthesis (cDNA)
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Purpose
- EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.
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Brand
- EasyScript®
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Specificity
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Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb. -
Characteristics
- The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
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Components
- EasyScript® Reverse Transcriptase, 5xES RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
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Unit Definition
- One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.
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Application Notes
- First-strand cDNA synthesis, Multiple copy gene detection
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Restrictions
- For Research Use only
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Buffer
- 5xES RT Buffer: 375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
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Storage
- -20 °C
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Storage Comment
- at -20°C for two years
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Expiry Date
- 24 months
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