EasyTaq® DNA Polymerase (with 2.5 mM dNTPs)
PCR
Quick Overview for EasyTaq® DNA Polymerase (with 2.5 mM dNTPs) (ABIN5519437)
Application
Polymerase Chain Reaction (PCR)
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Purpose
- EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
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Brand
- EasyTaq®
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Specificity
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. It lacks 3'-5' exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
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Characteristics
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- Extension rate is about 1-2 kb/min.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 4 kb. -
Components
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
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Unit Definition
- One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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Application Notes
- Routine PCR,Colony PCR
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Comment
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EasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
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Restrictions
- For Research Use only
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Buffer
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Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers.
10xEasyTaq® Buffer (with Mg2+): 200 mM Tris-HCl ( pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others. -
Storage
- -20 °C
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Storage Comment
- at -20°C for two years
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Expiry Date
- 24 months
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