Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
343 Products
- Application:
- Polymerase Chain Reaction, DNA Amplification
- Components:
- Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
- 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
- MgCl2 solution: 25 mM MgCl2
- Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme that incorporates, 10nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
- Specificity:
- Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188324
$140.12
Plus shipping costs $45.00
Delivery in 6 to 7 Business Days
Delivery in 6 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction, DNA Amplification
- Components:
- Red Diamond Taq® is provided at a concentration of 5 U/μl with 10x reaction buffer and MgCl2 solution.
- 10x Reaction buffer: 750 mM Tris-HCl, 200 mM (NH4)2SO4, 0.1 % (v/v) Tween 20 and stabilizer, pH 8.8 (at 19 °C).
- MgCl2 solution: 25 mM MgCl2
- Enzyme storage buffer: 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer pH 8.0 (19 °C)
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of enzyme that incorporates, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
- Specificity:
- Red Diamond Taq® polymerase* is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5'→3' synthesis of DNA with no detectable 3'→5' proof reading exonuclease activity. This enzyme has the "extendase" activity allowing TA cloning.
Catalog No. ABIN3188327
$140.12
Plus shipping costs $45.00
Delivery in 6 to 7 Business Days
Delivery in 6 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of TaqFast DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219168
$143.97
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of TaqFast DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219167
$143.97
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Unit Definition:
- One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666441
$145.00
200 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Protein Extraction
- Storage:
- 4 °C
- Unit Definition:
- According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Catalog No. ABIN6383963
$149.00
Plus shipping costs $45.00
Delivery in 11 to 13 Business Days
Delivery in 11 to 13 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- RNA Modification
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Thermostable RNase H that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 45°C [optimal temperature for poly(rA):poly(dT)] in 1X Thermostable RNase H Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Catalog No. ABIN3188239
$150.44
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Amplification
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
$150.44
500 U
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of Bloodirect DNA Polymerase in this MasterMix offers:
- Elimination of lengthy DNA purification steps
- Direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219177
$150.44
5 mL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519442
$152.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- 5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519502
$155.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- 5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519521
$155.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 5X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519536
$155.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- Taq DNA Polymerase 5 U/μl, 10xTaq PCR Buffer, 25 mM MgCl2
- Unit Definition:
- One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666457
$155.00
2 x 500 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
Catalog No. ABIN3188318
$163.85
335 μL
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 6 to 7 Business Days
Delivery in 6 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- 4 °C,-20 °C
Catalog No. ABIN5666448
$170.00
5 mL
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- 2xTaq PCR Buffer
0.2 U/μl Taq DNA Polymerase
0.6 mM each dNTPs
6 mM MgCl2
PCR enhance - Storage:
- 4 °C,-20 °C
Catalog No. ABIN5666459
$170.00
5 mL
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- cDNA Synthesis
- Components:
- TransScript® RT/RI Enzyme Mix, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb.
Catalog No. ABIN5519500
$174.00
Plus shipping costs $45.00 and $22.60 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- 4 °C,-20 °C
Catalog No. ABIN5666444
$174.00
5 mL
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Service
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