Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
329 Products
- Application:
- RNA Modification
- Concentration:
- 1 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
Catalog No. ABIN3188207
$137.50
100 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- cDNA Synthesis
- Components:
- EasyScript® RT/RI Enzyme Mix, 2xES Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 8 kb.
Catalog No. ABIN5519428
$141.69
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of TaqFast DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219168
$143.97
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of TaqFast DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219167
$143.97
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Protein Extraction
- Storage:
- 4 °C
- Unit Definition:
- According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Catalog No. ABIN6383963
$149.00
Plus shipping costs $45.00
Delivery in 11 to 13 Business Days
Delivery in 11 to 13 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Ligation
- Concentration:
- 5 U/μL
- Storage:
- -80 °C
- Specificity:
- An enzyme which anneals DNA strands together
Catalog No. ABIN411759
$150.00
250 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 5X Pfu Buffer, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519533
$150.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- The use of Bloodirect DNA Polymerase in this MasterMix offers:
- Elimination of lengthy DNA purification steps
- Direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219177
$150.44
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Amplification
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
$150.44
500 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Catalog No. ABIN5519442
$152.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- 5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519502
$153.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- 5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Catalog No. ABIN5519521
$153.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Components:
- DNA Polymerase, 5X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
- Specificity:
- TransStart® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
Catalog No. ABIN5519536
$153.00
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 15 to 19 Business Days
Delivery in 15 to 19 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Unit Definition:
- One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666442
$155.00
500 U
Plus shipping costs $45.00
Delivery in 2 to 3 Business Days
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Restriction Digest
- Concentration:
- 20000 U/mL
- Components:
- NotI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
- Storage:
- -20 °C
Catalog No. ABIN3188202
$156.91
10000 U
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
Catalog No. ABIN3188318
$159.50
335 μL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 6 to 7 Business Days
Delivery in 6 to 7 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- Robust PCR performance
- High resistance to most PCR inhibitors commonly found in samples, including plant samples
- 2X MasterMix format for easy PCR setup
- Advanced formulation for MasterMix Stability
- MasterMix-With Dye available for easy, direct gel loading
Catalog No. ABIN4219110
$176.32
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- cDNA Synthesis
- Components:
- cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
- Storage:
- -20 °C
- Specificity:
- - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
Publications
- Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Catalog No. ABIN4219170
$176.32
100 x 20 μL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219175
$176.32
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix. This PCR MasterMix contains loading dye. It allows direct gel electrophoresis of PCR products without the need of adding loading dye to finished PCR products.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Catalog No. ABIN4219176
$176.32
5 mL
Plus shipping costs $45.00 and $22.00 dry ice
Delivery in 3 to 4 Business Days
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Service
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