Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
- Application:
- cDNA Synthesis
- Components:
- TransScript® All-in-One SuperMix for PCR, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- One-tube format for simple and fast setup and reduce pipetting variability.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 12 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- 5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- DNA Modification
- Concentration:
- >2000 Kunitz U/mg
- Storage:
- -20 °C
Delivery in 11 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- EasyScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Amplification of fragment up to 4 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- DNA Manipulation
- Concentration:
- 5 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of Bsu DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 °C.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- 5xTransScript® All-in-One SuperMix for PCR, 2xTransTaq® HiFi PCR SuperMix II, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Amplification of fragment up to 12 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- TransScript® II Reverse Transcriptase, 5xTS II RT Buffer, Anchored Oligo (dT)20 Primer (0.5 μg/μL)
- Storage:
- -20 °C
- Unit Definition:
- One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
- Specificity:
- Increased thermostability for more full-length cDNA products.
Reaction temperature at 42 °C-55 °C.
Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Components:
- EvoTM Reverse Transcriptase (200 U/μl), 5X RT buffer
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- DNA Modification
Delivery in 11 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- gDNA remover and 5xTransScript® All-in-One SuperMix for qPCR are provided for simultaneous gDNA removal and cDNA synthesis.
TransStart® Tip Green qPCR SuperMix is provided for qPCR.
5xTransScript® All-in-One No-RT Control SuperMix for qPCR is provided for experimental control.
Passive reference dyes are provided for different qPCR instruments.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)