Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
- Components:
- EvoTM Reverse Transcriptase (200 U/μl), 5X RT buffer
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- TransScript® One-Step Enzyme Mix, 2x One-Step Reaction Mix, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Amplification of fragment up to 8 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- RNA Modification
- Concentration:
- 10 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of RNase R that converts 1 μg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- 5x TransScript® All-in-One SuperMix for PCR, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
cDNA up to 15 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Polymerase Chain Reaction
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Components:
- NovoTM Reverse Transcriptase (200 U/μl), 5X RT Buffer
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- C to C Lysis Buffer, Enzyme Mix, gDNA Remover, Reaction Mix, Oligo (dT)/RP Mix, RNase-free Water
- Storage:
- -20 °C
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- TransScript® II RT/RI Enzyme Mix, gDNA Remover, 2xTS II Reaction Mix, Random Primer (0.1 μg/μL), Anchored Oligo(dT)20 Primer (0.5 μg/μL), RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 15 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- 5xTransScript® II All-in-One SuperMix for PCR, 5xTransScript® II All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- 5xTransScript® II All-in-One SuperMix for PCR, RNase-free Water
- Storage:
- -20 °C
- Specificity:
- One-tube format for simple and fast setup and reduced pipetting variability.
The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
PCR ready cDNA in 30 minutes (unsuitable for qPCR).
cDNA up to 15 kb.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- cDNA Synthesis
- Components:
- Enzyme Mix, Reaction Mix, Universal miRNA qPCR Primer (10μM), RNase-free Water
- Storage:
- -20 °C
- Specificity:
- Optimized enzyme and buffer system for high efficient cDNA synthesis.
One-step Poly(A) tailing and cDNA synthesis.
Delivery in 12 to 16 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Components:
- Reaction Mix (4X), Reaction Stopper (5X), 5X All-In-One RT Mastermix, Nuclease-free H2O
- Storage:
- -20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Components:
- Jade ExpressTM 2X QPCR Master Mix is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Components:
- Jade ExpressTM Mix-Low ROX is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Components:
- Jade ExpressTM Mix-ROX 2X QPCR mixture is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Components:
- Jade ExpressTM 2X Master Mix-iCycler is a 2X concentrated mix of TaqFast DNA Polymerase, dNTPs, MgCl2, fluorescent dye (detection), reference dye and proprietary buffer components.
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- Application:
- Quantitative real-time PCR
- Components:
- Jade SmartTM 2X QPCR Master Mix is a 2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, fluorescent detection dye, reference dye, and proprietary buffer components.
- Storage:
- 4 °C,-20 °C
Delivery in 2 to 3 Business Days
- Order hotline:
- +1 877 302 8632 (TF)
- +1 888 205 9894 (TF)
- antibodies-online
- antikoerper-online.de (deutsch)
- antibodies-online.com (english)
- anticorps-enligne.fr (français)
- antibodies-online.cn (中文)
- genomics-online
- genomics-online.com (english)