Enzymes

Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.

Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.

368 Products
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    Enzyme
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
-20 °C
Specificity:
The use of TaqFast DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by 80 %
Catalog No. ABIN4219168
€127.41
5 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Golden qPCR TaqMan Probe Master Mix(2x)is a special mixture for Taqman probe real time PCR Which includes the hot-start DNA polymerase, PCR Buffer, MgCl2, dNTPs and all other components required for quantitative real-time PCR, except DNA template, primers and probes.

Application:
Quantitative real-time PCR
Storage:
4 °C,-20 °C
Catalog No. ABIN5666448
€128.32
5 mL
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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2xTaq PCR Master Mix is an optimal 2x concentrated solution of Taq DNA polymerase, dNTPs, MgCl2 and all other components required for PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity. The PCR products generated using this product contain dA overhangs at the 3'-end, and can be ligated to dT/-overhang vectors easily.

Application:
Polymerase Chain Reaction
Components:
2xTaq PCR Buffer
0.2 U/μl Taq DNA Polymerase
0.6 mM each dNTPs
6 mM MgCl2
PCR enhance
Storage:
4 °C,-20 °C
Catalog No. ABIN5666459
€128.32
5 mL
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Binuclease is a genetically engineered endonuclease from Serratia marcescens gene that can digest all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester.

Application:
Protein Extraction
Storage:
4 °C
Unit Definition:
According to the protocol from Sigma-Aldrich, one unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 at 37°C.
Catalog No. ABIN6383963
€131.86
50 kU
Plus shipping costs €45.00
Delivery in 11 to 13 Business Days
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Golden qPCR SYBR Green Master Mix(2x) is an optimal 2x concentrated solution of hot-start DNA polymerase, MgCl2, dNTPs, SYBR Green I dye and all other components required for quantitative real-time PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity.

Application:
Quantitative real-time PCR
Storage:
4 °C,-20 °C
Catalog No. ABIN5666446
€131.86
5 mL
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Golden qPCR EVA Green Master Mix(2x) is an optimal 2x concentrated solution of hot-start DNA polymerase, dNTPs, EVA Green dye, ROX and all other components required for quantitative real-time PCR, except DNA template and primers. This is a premixed, ready-to-use solution with high sensitivity and specificity.

Application:
Quantitative real-time PCR
Storage:
4 °C,-20 °C
Catalog No. ABIN5666444
€131.86
5 mL
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Application:
RNA Modification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of Thermostable RNase H that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 45°C [optimal temperature for poly(rA):poly(dT)] in 1X Thermostable RNase H Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).
Catalog No. ABIN3188239
€133.13
500 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Application:
DNA Amplification
Concentration:
5 U/μL
Components:
Enzyme supplied with 10X Reaction Buffer
Storage:
-20 °C
Unit Definition:
One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
Catalog No. ABIN3188210
€133.13
500 U
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Streamline your PCR with 2X PCR MasterMix

Application:
Polymerase Chain Reaction
Concentration:
2 x
Storage:
-20 °C
Specificity:
The use of Bloodirect DNA Polymerase in this MasterMix offers:
- Elimination of lengthy DNA purification steps
- Direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Catalog No. ABIN4219177
€133.13
5 mL
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 3 to 4 Business Days
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Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis.

Application:
cDNA Synthesis
Components:
TransScript® RT/RI Enzyme Mix, gDNA Remover, 2xTS Reaction Mix, Random Primer (N9) (0.1 μg/μL), Anchored Oligo (dT)18 Primer (0.5 μg/μL), RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
The product obtained from 15 minutes reaction is used for qPCR, the product obtained from 30 minutes reaction is used for PCR.
cDNA up to 12 kb.
Catalog No. ABIN5519519
€136.28
50 reactions
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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RNase A is ribonuclease that cleaves a single-stranded RNA.

Application:
RNA Modification
Catalog No. ABIN2953736
€134.83
1 mL
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952628
€134.83
250 U
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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Dnase I

Application:
DNA Modification
Concentration:
>2000 Kunitz U/mg
Catalog No. ABIN2952934
€146.07
15000 U
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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Deoxyribonuclease I

Application:
DNA Modification
Catalog No. ABIN2952656
€146.07
20 mg
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins.

Application:
Polymerase Chain Reaction
Components:
DNA Polymerase (with 2.5 mM dNTPs)
Storage:
-20 °C
Specificity:
Functional absence of double and single stranded endonuclease activity.
Catalog No. ABIN2952629
€146.07
250 U
Plus shipping costs €45.00
Delivery in 11 to 16 Business Days
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The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA.

Application:
cDNA Synthesis
Components:
5xTransScript® All-in-One SuperMix for PCR, 5xTransScript® All-in-One No-RT Control SuperMix for qPCR, gDNA Remover, RNase-free Water
Storage:
-20 °C
Specificity:
Simultaneous genomic DNA removal and cDNA synthesis.
The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
qPCR ready cDNA in 15 minutes.
cDNA up to 250 bp.
Catalog No. ABIN5519498
€149.56
50 reactions
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 15 to 19 Business Days
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FlashPfu is a DNA polymerase for high-fidelity PCR. By fusing Pfu with a high affinity double-stranded DNA binding domain, we have dramatically increased its processivity and extension rate. FlashPfu DNA polymerase is 50-fold more accurate than Taq polymerase and 6-fold more accurate than Pfu polymerase.

Application:
Polymerase Chain Reaction
Components:
FlashPfu DNA Polymerase 1 U/μl, 2x flashPfu PCR Buffer, 25 mM MgSO4
Unit Definition:
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid insoluble material in 30 minutes at 75°C.
Catalog No. ABIN5666439
€149.56
250 U
Plus shipping costs €45.00
Delivery in 2 to 3 Business Days
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Cas9 (D10A) NLS Protein

Application:
Genome Editing with Engineered Nucleases
Components:
Cas9 (D10A) NLS, 10X Cas9 Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN5684188
€152.21
50 pmol
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 2 to 3 Business Days
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Cas9 (H840A) NLS Protein

Application:
Genome Editing with Engineered Nucleases
Components:
Cas9 (H840A) NLS, 10X Cas9 Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN5684190
€152.21
50 pmol
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 2 to 3 Business Days
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Cas9 Null NLS Protein

Application:
Genome Editing with Engineered Nucleases
Components:
Cas9 Null NLS, 10X Cas9 Reaction Buffer
Storage:
-20 °C
Catalog No. ABIN5684200
€152.21
50 pmol
Plus shipping costs €45.00 and €20.00 dry ice
Delivery in 2 to 3 Business Days