Enzymes
Enzyme describes a broad spectrum of biocatalysts that carry out nearly all of the molecular chemistry necessary for life. Most enzymes are single or multi-subunit proteins that recognize a single substrate, and reduce the activation-energy necessary to convert that substrate into a specific product. Enzymes are particularly useful tools in the molecular biologist’s tool kit. They are widely used in both genomics and proteomics research.
Many different classes of enzymes are used to manipulate genetic sequences. E.g. restriction endonucleases cleave DNA at a specified sequence, polymerases amplify a double stranded nucleotide sequence, and ligases join nucleotide sequences together. Recently, engineered restriction nucleases like Cas9 have gathered significant research interest, as they may be used for targeted excision and replacement of genomic DNA –allowing a simple method for “on-the-fly” editing of a specific gene. We offer a wide variety of enzymes, restriction enzymes, polymerases and more products for different applications. For questions regarding our product portfolio, please contact our customer service via chat, phone, or email.
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The ready-to-use PCR MasterMix is a proprietary mixture of high quality DNA Polymerase, dNTPs, Mg2+, and PCR buffer in a 2X concentration. It contains all necessary reagents for DNA amplification. To set up a PCR, simply add DNA template, primers, and nuclease-free H2O to yield a 1X reaction mix. This PCR MasterMix contains loading dye. It allows direct gel electrophoresis of PCR products without the need of adding loading dye to finished PCR products.
The use of Bestaq™ DNA Polymerase in this MasterMix offers:
- High-speed PCR without compromising accuracy
- High-processivity to reduce reaction time by up to 70 %
- Robust and high yield across a wide range of templates
- Efficient amplification of DNA templates up to 15kb
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction, Multiplex Assay
- Concentration:
- 2 x
- Components:
- 5 x 1.0 ml vials
- Storage:
- -20 °C
- Specificity:
- With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction, Multiplex Assay
- Concentration:
- 2 x
- Components:
- 5 x 1.0 ml vials
- Storage:
- -20 °C
- Specificity:
- With HotStart DNA Polymerase, you can achieve
- The highest specificity with minimal background
- Superior performance
- Improved yield of desired product
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- Robust PCR performance
- High resistance to most PCR inhibitors commonly found in samples, including plant samples
- 2X MasterMix format for easy PCR setup
- Advanced formulation for MasterMix Stability
- MasterMix-With Dye available for easy, direct gel loading
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- cDNA Synthesis
- Components:
- cDNA Synthesis SuperMix is a proprietary mixture of all the materials required for first-strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix.
- Storage:
- -20 °C
- Specificity:
- - Streamlined protocol suitable for high-throughput applications
- Ease of use with a simple set-up
- Excellent cDNA yield
- This SuperMix contains abm's EasyScript™ Reverse Transcriptase.
- Kim, Yoon, Kim, Noh, Kwon, Yi, Lee, Choi, Kang, Park, Lee, Ye: "Alleviation of collagen-induced arthritis by the benzoxathiole derivative BOT-4-one in mice: Implication of the Th1- and Th17-cell-mediated immune responses." in: Biochemical pharmacology, Vol. 110-111, Issue , pp. 47-57, 2016 (Pubmed)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Concentration:
- 2 x
- Storage:
- -20 °C
- Specificity:
- Robust PCR performance
- High resistance to most PCR inhibitors commonly found in samples, including plant samples
- 2X MasterMix format for easy PCR setup
- Advanced formulation for MasterMix Stability
- MasterMix-With Dye available for easy, direct gel loading
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
Delivery in 11 to 16 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction
- Storage:
- -20 °C
- Specificity:
- Functional absence of double and single stranded endonuclease activity.
Delivery in 11 to 16 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction, Mutagenesis
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The use of Precision™ DNA Polymerase in this MasterMix offers:
- Outstanding accuracy and efficiency
- Error-free PCR products for downstream processes
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- cDNA Synthesis
- Concentration:
- 200 U/μL
- Storage:
- -20 °C
- Specificity:
- EasyScript PlusTM is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. EasyScript PlusTM is engineered to perform under high temperatures (50 °C - 55 °C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, EasyScript PlusTM can synthesize full-length cDNA libraries from RNA templates up to 15 kb in length. In addition, EasyScript PlusTM has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Polymerase Chain Reaction, Mutagenesis
- Concentration:
- 2 x
- Storage:
- 4 °C/-20 °C
- Specificity:
- The use of Precision™ DNA Polymerase in this MasterMix offers:
- Outstanding accuracy and efficiency
- Error-free PCR products for downstream processes
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- DNA Modification
- Concentration:
- 10 U/μL
- Components:
- Enzyme supplied with 10X Reaction Buffer
- Storage:
- -20 °C
- Unit Definition:
- One unit is defined as the amount of T7 Endonuclease I that linearizes over 90% of 1 μg of supercoiled cruciform DNA in a 50 μl volume in 1 hour at 37°C.
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Ligation
- Concentration:
- 200 U/μL
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Components:
- Each EvaGreen 2X qPCR MasterMix is a 2X mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, reference dye and proprietary buffer components. Each set of EvaGreen qPCR MasterMix contains 4 vials, each containing 1.25 ml.
- Storage:
- -20 °C
- Specificity:
- qPCR Instruments:
- BioRad® iCycler®
- iQ™5
- MyiQ™
- Chi, Bhagwat, Tang, Xiang: "Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs." in: Methods in molecular biology (Clifton, N.J.), Vol. 1405, Issue , pp. 163-78, 2016 (Pubmed)
- Lee, Lee, Shim: "Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane." in: Biochimica et biophysica acta, Vol. 1864, Issue 2, pp. 303-313, 2016 (Pubmed)
- Gao, Yang, Zhang, Chen, Tian: "Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark." in: BMC plant biology, Vol. 16, Issue 1, pp. 231, 2016 (Pubmed)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- ChIP DNA-Sequencing, Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- Quantitative real-time PCR
- Storage:
- -20 °C
- Su, Xu, Guan, Wang, Wu, Zhai, Chen, Hu: "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." in: International immunopharmacology, Vol. 38, Issue , pp. 16-25, 2016 (Pubmed)
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- ChIP DNA-Sequencing, Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)
- Application:
- ChIP DNA-Sequencing, Quantitative real-time PCR
- Storage:
- -20 °C
- Specificity:
- - Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Delivery in 3 to 4 Business Days
- Order hotline:
- +1 877 302 8632
- +1 888 205 9894 (Toll-free)