MutaPLATE® HLA-DQ2+8 (TM) real time PCR Kit contains three allele specific primers and TaqMan probes, labeled with FAM, for the detection of the HLA-DQ2 & DQ8 alleles. The TaqMan probes bind to the amplificated target-DNA. Due to this, a fluorescence signal is generated and detected by the optical unit of the real time PCR instrument (e. g. Light Cycler®, Roche). Positive samples are identified due to the increase in fluorescence on the FAM (530 nm) channel during the PCR. An internal amplification control (IC), labeled with Yakima Yellow (560 nm), is also amplified with every sample. For a valid result, there must be a successful amplification of the IC on the YAK channel.