Tris-Borate-EDTA TBE Buffer (10X)

Details for Product No. ABIN925559,
Agarose Gel Electrophoresis (AGE)
Characteristics TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.
Synonyms: buffer containing boric acid and EDTA
Sterilization: Sterile Aseptic Filter
Application Notes This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3.  Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Restrictions For Research Use only
Format Liquid
Concentration 10 X
Storage RT